E.coli can make Disulphide bonds if you ask it nicely.

Human protein, expressed in NEB SHuffle cells, correctly folded, crystallised, solved, etc.

Electron density around a Disulphide bond – which E.coli cannot make in their cytoplasm normally – unless you tinker with a few genes here and there.

The Disulphide is the bond between the two green sulphur atoms in the centre of the image

EDIT: Click to see the picture more clearly – I don’t know what WP has done to it, but it should look crisper than that.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s

%d bloggers like this: